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Research ArticleResearch Articles
Open Access

Direct Seeding Asclepias humistrata (Sandhill Milkweed) in Coastal Dunes

Gabriel Campbell-Martínez, Mack Thetford and Debbie Miller
Ecological Restoration, May 2025, 43 (2) 116-125; DOI: https://doi.org/10.3368/er.43.2.116
Gabriel Campbell-Martínez
University of Florida, West Florida Research and Education Center, 5988 Highway 90, Building 4900, Milton, FL 32583, USA
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  • For correspondence: Camp5595{at}gmail.com
Mack Thetford
University of Florida, West Florida Research and Education Center, Milton, FL, USA
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Debbie Miller
University of Florida, West Florida Research and Education Center, Milton, FL, USA
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    Figure 1

    Microsites (A) under the scrub oak canopy of Quercus geminata (sand live oak) or Q. myrtifolia (myrtle oak), (B) on the gulf-side scrub oak edge (drip line of Quercus), or (C) in open gaps of bare sand within coastal scrub located in coastal Alabama.

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    Figure 2

    Protection treatments included a control with no protection, protection with a PVC pipe (7.6 cm length and 10.2 cm diameter), protection with a wire mesh (1.27 cm hardware cloth), and protection with both a PVC pipe and wire mesh.

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    Figure 3

    Emergence of Asclepias humistrata (N=40) over 52 weeks after sowing on June 9, 2019 in three planting microsites (seeds sown under a scrub oak canopy of Quercus geminata (sand live oak) or Q. myrtifolia (myrtle oak), on the gulf-side edge (drip line) of coastal scrub or in an open area gulf-side of coastal scrub) with seeds covered by leaf litter (dashed lines) or with sand only (solid lines).

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    Asclepias spp. (milkweed) flower showing pollination. The pollen coheres into twin waxy masses (pollinia, image b), and the handle or caudicle lies in a chink on the side of the stigma. L.H. Bailey, 1917. Standard Cyclopedia of Horticulture. New York, NY: The MacMillan Company. The Florida Center for Instructional Technology, fcit.usf.edu.

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    Table 1

    Type III Tests of Fixed Effects and their interactions on seedling emergence (%) for Asclepias humistrata evaluated over 52 weeks in response to sowing seed within three microsite conditions, with two types of seed cover and four levels of protection. Weeks after Sowing = Seed sown June 9, 2019 and evaluated for emergence after 6, 7, 11, 20, 23, 38, and 52 weeks; Planting microsite = (seeds sown under a scrub oak canopy of Quercus geminata (sand live oak) or Q. myrtifolia (myrtle oak), on the gulf-side edge (drip line) of coastal scrub or in an open area gulf-side of coastal scrub); Seed cover = seeds covered by leaf litter or covered only with sand; Planting site protection = (seeds planted in open sand without protection, within PVC pipes, covered with a wire mesh or within PVC pipes covered with a wire mesh cover.

    EffectNum DFDen DFF ValuePr > F
    Weeks after sowing (WAS)666826.68<0.0001
    Microsite26720.010.9874
    WAS × Microsite1266814.12<0.0001
    Cover166878.33<0.0001
    WAS × Cover66680.780.5827
    Microsite × Cover26684.530.0112
    WAS × Microsite × Cover126683.220.0002
    Protection366816.44<0.0001
    WAS × Protection186681.170.2835
    Microsite × Protection66680.890.5049
    WAS × Microsite × Protection366680.640.9492
    Cover × Protection36686.100.0004
    WAS × Cover × Protection186680.480.9650
    Microsite × Cover × Protection66680.760.5997
    WAS × Microsite × Cover × Protection366680.420.9990
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    Table 2

    Seedling emergence (%) of Asclepias humistrata in response to planting microsite (seeds sown under a scrub oak canopy of Quercus geminata (sand live oak) or Q. myrtifolia (myrtle oak), on the gulf-side edge (drip line) of coastal scrub or in an open area gulf-side of coastal scrub) and seed cover (seeds covered by leaf litter or covered only with sand) 7 and 38 weeks after sowing (WAS).

    Microsite7 WASz38 WAS
    SandLitterSandLitter
    Open1.50 by0.00 b20.50 a9.75 bc
    Edge3.00 b1.75 b14.00 ab11.50 bc
    Canopy16.75 a2.50 b6.00 bc3.75 c
    • ↵z Seeds sown June 9, 2019 (n=40), emergence evaluated 7 WAS (July 27, 2019) and 38 WAS (March 6, 2020).

    • ↵y Means separation within a date (WAS) by Tukey-Kramer Least Squares Means; sand and litter means within a date followed by the same letter do not differ (Alpha=0.05).

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    Table 3

    Effects of microsite, seed cover, and protection on growth characteristics of Asclepias humistrata (sandhill milkweed) seedlings 52 weeks after direct seeding into backdunes of coastal Alabama on 6/9/2019. Seeds were placed in a microsite under a scrub oak canopy of Quercus geminata (sand live oak) or Q. myrtifolia (myrtle oak), on the gulf-side edge (drip line) of coastal scrub or in an open area gulf-side of coastal scrub. Seeds were covered with 2 cm of sand or nearby litter (Quercus leaves). Seeds were either unprotected or protected with PVC pipes (7.6 cm length and 10.2 cm diameter) buried to ½ their height above ground, protected with 1.27 cm wire-mesh that was bent to form square cages positioned approximately 3.5 cm above seeds, or protected with both pvc and wire mesh cages.

    TreatmentNHeight (cm)Mean Width (cm)Leaf length (cm)Nodes (# per plant)
    Microsite
    Canopy131.8±0.231.2±0.091.1±0.082.2±0.37
    Edge631.4±0.071.0±0.011.0±0.022.1±0.08
    Open1061.4±0.051.0±0.021.0±0.022.2±0.06
    Soil Cover
    Litter611.7±0.081.0±0.031.0±0.022.0±0.10
    Sand1211.3±0.041.0±0.011.0±0.022.2±0.06
    Protection
    Control341.2±0.071.0±0.011.0±0.002.3±0.12
    PVC281.3±0.101.0±0.021.0±0.042.0±0.11
    Mesh611.4±0.071.0±0.031.0±0.021.9±0.07
    PVC + Mesh591.6±0.091.0±0.021.1±0.032.3±0.10

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Ecological Restoration: 43 (2)
Ecological Restoration
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1 May 2025
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Direct Seeding Asclepias humistrata (Sandhill Milkweed) in Coastal Dunes
Gabriel Campbell-Martínez, Mack Thetford, Debbie Miller
Ecological Restoration May 2025, 43 (2) 116-125; DOI: 10.3368/er.43.2.116

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Direct Seeding Asclepias humistrata (Sandhill Milkweed) in Coastal Dunes
Gabriel Campbell-Martínez, Mack Thetford, Debbie Miller
Ecological Restoration May 2025, 43 (2) 116-125; DOI: 10.3368/er.43.2.116
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